Journal: Infection and Immunity

Article Title: Vaccination against the Intracellular Pathogens Leishmania major and L. amazonensis by Directing CD40 Ligand to Macrophages

doi: 10.1128/iai.69.5.3255-3263.2001

Figure Lengend Snippet: FIG. 4. The expression of antigens on the surface of transfected L929 cells. Transfected L cells were incubated with MR1 MAb to CD40L or MAb #96 to Leishmania gp63 and were stained with FITC-conjugated anti-IgG, as described in Materials and Methods. After being extensively washed, cells were fixed in 1% paraformaldehyde in PBS and analyzed on an Epics Elite flow cytometer. Profiles of cells stained with each antibody were overlaid and compared to those of cells stained with secondary antibody alone.

Article Snippet: After 45 min at 4°C in the primary antibody, the cells were washed with cold buffer and incubated with either fluorescein isothiocyanate (FITC)-conjugated goat anti-hamster IgG (Jackson ImmunoResearch, West Grove, Pa.) for CD40L or FITC-conjugated goat anti-mouse IgG (Jackson ImmunoResearch) for gp63 for 45 min at 4°C.

Techniques: Expressing, Transfection, Incubation, Staining, Cytometry